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Microbiology

Location: 
5th Floor VMMC Building 
Clinical Laboratory ICMR Building

Guidelines for Antimicrobial Therapy-2011 
Guidelines for Infection Control Policy

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Investigation (Microbiology)

  • Bacteriology - Staining & Microscopy, Aerobic & Anaerobic culture of blood, CSF, urine, pus, respiratory & other body fluids. Antimicrobial susceptibility testing and MIC testing by Etest. Rapid test for pyogenic meningitis.
  • Immunodiagnostics - ASLO, CRP, RF, Widal
  • Mycology - Direct microscopy of various samples for fungal elements, special stains & culture, Antigen detection & antifungal susceptibility of yeasts and molds (Aspergillus species) , PAN fungal PCR .
  • TB - DOTS lab management, Microscopy, culture and sensitivity, first and second line ATT, accuprobe AMTDT and real time PCR for extrapulonary TB, quartiferon TB gold.
  • Virology - ELISA for Dengue, Chikungunya, CMV, Herpes, Rubella.
  • Parasitology - Serology for Leishmaniasis (rk – 39), filaria, amoebiasis, hydatid disease, toxoplasma (IgG, IgM), Neuro cryptococcosis, Microscopy for malaria, filaria, Stool for ova – cyst.

Timing:

Laboratory is open from 9.00 AM to 4.00 PM (Monday to Friday) and from 9.00 AM to 1.00 PM (Saturday). Emergency services are provided on Sunday and Holiday (9.00 AM to 12.00 Noon). 
Samples are received till 12.00 Noon. However, emergency samples are received till 3.00 PM on working days.

Reports:

Reports are released and distributed in ward & OPD’s as and when ready.

Other Activities:

Infection Control: 
Department conducts prevalence survey annually to monitor Hospital Acquired Infection rates in addition to laboratory based surveillance of pathogens & their antibiotic resistance patterns.

Investigations:

  • Environment culture
  • Water culture
  • Tracing and monitoring outbreaks

Sr. No. Name of Faculty Designation Email
1 Dr. Monorama Deb Director Professor
2 Dr. Rajini Gaind Consultant Head of the Department
3 Dr. Balvinder Singh Arora Professor
4 Dr. Jagdev Kaur Senior Specialist
5 Dr. Deepthi Nair Senior Specialist
6 Dr. Dimple Kasana Senior Specialist
7 Dr. Malini R. Capoor Specialist
8 Dr. Sarika Jain Assistant Professor
9 Dr. Sarita Mohapatra Assistant Professor
10 Dr. Tripta Kaur Assistant Professor
11 Dr. Ayush Gupta Assistant Professor
12 Dr. Sudhir Kumar Bagdi Chief Medical Officer

At present there is no content available for this section, once content will be available would be updated.

At present there is no content available for this section, once content will be available would be updated.

  • ICMR Project “ Effect of Probiotic on Prevention of sepsis in LBW infants during 0-2 month period: A Randomized controlled trial.” ( Principal Investigator) 2008-2011 Completed
  • ICMR : To Study genetic diversity of Fluoroqinolone resistant Salmonella enterica serovar Typhi and Paratyphi A.( Principal Investigator) 2010-2013 ongoing
  • ICMR: Phenotypic detection and molecular characterization of carbapenemase-mediated resistance among nosocomial bloodstream isolates of Escherichia coli and Klebsiella pneumonia ( Principal Investigator ) 2012-2014 ongoing
  • Multieccentric project on neonatal sepsis Registry - ICMR funded ( site PI) ongoing
  • Indo-Japanese TB project on- Study of Mycobacterium tuberculosis using population based genotyping novel diagnostic and therapeutic methods. International exchange collaboration of Department of Science and technology, Ministry of Science and Technology Govt. of India. 2010-11 ( Completed )
  • ICMR Fellowship “Establishing a role of molecular tests in the diagnosis of TBM in cerebrospinal fluid samples”, SRF, Miss Pratibha Sharma 2010-2013. Completed
  • Multicentric study on hospital based surveillance of invasive pneumococcal disease. Study centre. CMC Vellore project Privately funded by GSK. ( Completed)
  • ICMR Adhoc Reseach Project 2010-13 Principal Investigator: A clinico-mycological and molecular study of cryptococcosis in a tertiary-care set-up. Completed
  • DBT project: Principal investigator (SJH). A system biology and pathogenomics of Aspergillus flavus infections in India 2013-2015
  • Co-investigator: A multi-centric study of Candidemia cases in ICU: SIHAM National Candidemia Network
  • . Co-Principal Investigator in ICMR project on “Establishment of networking for Neisseria gonorrhoeae surveillance in India” at Department of Microbiology, AIIMS, New Delhi since 2010.
  • Co-Principal Investigator in DBT Project on “Development of DNA Biosensor for detection of Neisseria gonorrhoeae in clinical samples” at Department of Microbiology, AIIMS and National physical Laboratory, New Delhi, 2009-12.
  • Co-Investigator in DBT project on “Development of Point-of-Care Test using LAMP Technology for Diagnosis of Gonorrhoea” at Department of Microbiology, AIIMS, New Delhi in 2009-10.
  • NACO Operational Research Project on n “Aetio-epidemiological study of ulcerative STIs in a tertiary care hospital in Delhi” at Regional STD Centre, Safdarjang Hospital, New Delhi. 2010- 2011. Completed and submitted report to NACO.
  • Co-investigator in ICMR Project on “Assesment of role of host genetics in HIV-1 infection” being carried out at Department of Biochemistry, AIIMS, New Delhi.
  • Needle stick injuries Prevention in health care workers in ICU and other high risk area of Hospital- D. Kasana, D Nair, M Deb. “Safe Hands” Organization (AUSAID), Australia funded project 2010- 2011 (Completed )

Publications

In International Journals

  • Singh V, Bala M, Kakran M, Ramesh V. Comparative assessment of CDS, CLSI disc diffusion and Etest techniques for antimicrobial susceptibility testing of Neisseria gonorrhoeae: A six year study. BMJ Open. 2012;2(4):1-7.e000969. doi:10.1136/bmjopen-2012-000969
  • Bhargava A, Thakur A, Mishra B, Taneja J, Dogra V, Loomba. P. Patient satisfaction survey of microbiological tests done in G.B. Pant Hospital. International Journal of Health Care Quality Assurance. 2012; 25(7):555-64.
  • Bhargava A, Mishra B, Thakur A, Dogra V, Loomba P, Jain M. Acinetobacter meningitis in post neurosurgical patients: epidemiology, antimicrobial resistance, treatment options and risk factors that predict the outcome. Canadian Journal of Infection Control. 2012;27(3):153.
  • Muralidhar S, Taneja A, Ramesh V. Patient safety culture- Perception of health care workers in a tertiary care hospital. International Journal of Risk and Safety Medicine. 2012;24(4): 191-99.
  • Puri P, Ramesh V , Singh A, Muralidhar S, Capoor MR. Facial eruption in Human Immunodeficiency Virus (HIV)- seropositive patient. International Journal of Dermatology. 2012;51:777-79.
  • Kulkarni S, Bala M, Risbud A. Antimicrobial susceptibility testing, auxotyping and serotyping of Neisseria gonorrhoeae strains isolated in India. Sex Transm Dis. 2012; 39(3):188-90.
  • Andrabi R, Bala M, Kumar R, Wig N, Hazarika A, Luthra K. Neutralization of Tier-2 Viruses and Epitope Profiling of Plasma Antibodies from Human Immunodeficiency Virus Type 1 Infected Donors from India. PLoS ONE. 2012:7(8):e43704. doi:10.1371/journal.pone.0043704
  • Kulkarni S, Bala M, Sans S, Pandey S, Bhattacharya J, Risbud A. Mutations in gyrA and parC genes in quinolone resistant Neisseria gonorrhoeae isolates in India. Int J Antimicrob Agents. 2012;40(6):549-53.
  • Andrabi R, Kumar R, Bala M, Nair A, Biswas A, Wig A, et al. Production and characterization of human anti-V3 monoclonal antibodies from the cells of HIV-1 infected Indian donors. Virol J. 2012;9:196. doi:10.1186/1743-422X-9-196.
  • Lahra MM, Limnios EA, Dorji D, Abu BHM, Darussalam B, Guillard B, et al. Surveillance of antibiotic resistance in Neisseria gonorrhoeae in the WHO Western Pacific and South East Asian regions, 2010. Commun Dis Intell. 2012;36(1):95-100.
  • Chaudhary O, Bala M, Singh J, Hazarika A, Kumar R, Luthra K. Relative reduction of plasmacytoid dendritic cells with shift in TH1 to TH2 response in HIV-1 infected patients as compared to high risk and healthy north Indians. BMC Infect Dis. 2012;12(Suppl 1):O20.
  • Andrabi R, Bala M, Luthra K. Cross clade reactive plasma anti-V3 antibodies in human immunodeficiency virus type-1 infected individuals develop with time. BMC Infect Dis. 2012;12(Suppl 1):O19.
  • Andrabi R, Bala M, Kumar R, Hazarika A, Luthra K. Neutralization of tier-2 viruses and epitope profiling of plasma antibodies from subtype-C HIV-1 infected north Indians; Implications for MPER directed HIV-1 neutralization. BMC Infect Dis. 2012;12(Suppl 1):P24.
  • Sexually transmitted infections, Chugh TD, Gaind R. Clin Lab Med. 2012 Jun;32(2):143-58.
  • Genetic characterization of Chikungunya virus from New Delhi reveal emergence of a new molecular signature in Indian isolatesShrinet J, Jain S, Sharma A, Singh SS, Mathur K, Rana V, Bhatnagar RK, Gupta B, Gaind R, Deb M, Sunil S. Virol J. 2012 May 25;9(1):100. [Epub ahead of print]
  • Comparison of an Automated System with Conventional Identification and Antimicrobial Susceptibility Testing. Shalini Duggal, Rajni Gaind, Neha Tandon,3 Manorama Deb, and Tulsi Das Chugh. International Scholarly Research Network ISRN Microbiology Volume 2012, Article ID 107203, 4 pages. doi:10.5402/2012/107203
  • Jain S, Gaind R, Chugh TD. in vitro activity of vancomycin and daptomycin against clinical isolates of Staphylococcus aureus and Enterococci from India. International Journal of Antimicrobial Agents. Accepted for publication in Feb 2013. Manuscript ID No: IJAA-D-1-00096
  • Sarika Jain, Rajni Gaind, Kunj Bihari Gupta, Reetika Dawar, Deepak Kumar, Premila Paul, Raman Sardana, Manorama Deb.Yokenella Regensburger infection mimicking enteric fever: a case report from India. Accepted for publication in Journal of Medical Microbiology 2013. Manuscript ID No: JMM/2013/058669
  • Prevalence, phenotypic characterization and colistin susceptibilities of carbapenem resistant bloodstream isolates of Pseudomonas aeruginosa and Acinetobacter spp" S. Mohanty, Vijeta Maurya. Rajni gaind and M. Deb accepted JIDC, Manuscript no 2924
  • Oropharyngeal Candidiasis and Candida colonization and its correlation with CD4 + cell count and ART in HIV infected patients in northern India. Accepted JIDC manuscript ID#2801
  • Mohapatra S. Malaria vaccine: a myth or a reality. Annals of Tropical Medicine and Public Health (Accepted for publication, manuscript number ATMPH_62_12)
  • Mohapatra S. Does naked eye examination of QBC capillary tube give a clue for malaria diagnosis? Annals of Tropical Medicine and Public Health (accepted for publication, manuscript number ATMPH_155_12)
  • Sinha A, Singh G, Bhat AS, Mohapatra S, Gulati A, Hari P, Samantaray S, Dinda AK, Agarwal AK, Bagga A. Thrombotic microangiopathy and acute kidney injury following vivax malaria. Clinical and Experimental Nephrology 2012 July 4: DOI 10.1007/s10157-012-0656-9.
  • Xess I, Mohapatra S, Shivaprakash MR, Chakrabarti A, Benny GL, O'Donnell K, Padhye AA. Evidence implicating Thamnostylum lucknowense as an etiological agent of rhino-orbital mucormycosis. Journal of Clinical Microbiology. 2012: 50(4): 1491- 2.
  • Mohapatra S, Samantaray JC. Survival of a child with 70% parasitemia due to Plasmodium falciparum infection. The Pediatric Disease Infectious Journal. 2012: 31(7): 790.
  • Acremonium kilniense First case of Acremonium kiliense mycetoma in a New Delhi resident: A brief review. Journal de Mycologie Médicale / Journal of Medical Mycology, 2012 S. Agarwal, M.R. Capoor, V. Ramesh, R. Rajni, G. Khanna
  • Fungal infections IN BURNS Epidemiological and clinico-mycological profile of fungal wound infection from largest burn centre in asia. MR Capoor* 1, S Gupta 1, S Sarabahi 2, A Mishra 2, VK Tiwari, P Aggarwal. 2012 55: 181-8 Mycoses
  • Changing pattern of fungal infection in burn patients. Sarabahi S, Tiwari VK, Arora S, Capoor MR, Pandey A. Burns 2012 38: 520-8 (International)
  • Sarabahi S, Tiwari VK, Arora S, Capoor MR, Pandey AResponse to letter to the editor: 'epidemiology of fungal infection in burns. Burns Sep;38(6):943-4. Epub 2012 Mar 20. (International)
  • Sushruta Das, Malini R Capoor, Avninder Singh, V Ramesh. Disseminated histoplasmosis in apparently immunocompetent individual from north India: A case report and review. Medical Mycology 2013 March early on line
  • Malini R Capoor, Sonal Aggarwal, Sachin Yadav, Anil Kumar Saxena, V Ramesh. Trichosporon mucoides causing onychomycosis in an immunocompetent patient. Int J Dermatology 2013 March
  • Malini R Capoor. State of the globe: MRSA continues to survive and sustain. J Global Infect Dis 2013 (Jan – March)
  • Sarika Jain, Tulsi Das Chugh. Current trends of antimicrobial resistance among blood culture isolates of salmonella enterica at a tertiary care centre in new delhi with a perspective on treatment of enteric fever. Journal of Infections in developing Countries. Accepted for publication in Feb 2013. Manuscript ID No: ID3030
  • Jain S, Das S, Gupta N, Malik JN. Frequency of fungal isolation and antifungal susceptibility pattern of the fungal isolates from nasal polyps of chronic rhinosinusitis patients at tertiary care centre in north India. Med Mycol. 2013 Feb; 51(2):164-9.
  • Randhawa HS, Chowdhary A, Kathuria S, Roy P, Misra DS, Jain S, Chugh TD. Blastomycosis in India: report of an imported case and current status. Med Mycol. 2013 Feb;51(2):185-92.
  • Kashyap B, Das S, Jain S, Agarwal A, Kaushik JS, Kaur IR. Correlation between the clinic-radiological heterogeneity and the immune-inflammatory profiles in pediatric patients with neurocysticercosis from a tertiary referral centre. J Trop Pediatr 2012; 58(4): 320-3.
  • Toolbox for tuberculosis diagnosis: an indian multicentric study (2006-2008) - microbiological results. Philippe h lagrange1*, satheesh k thangaraj2, rajeshwar dayal3, alka despande4, nirmal k ganguly5, enrico girardi6, beenu joshi7, kiran katoch7, vishwa m katoch7, manoj kumar8, vemu lakshmi9, marc leportier2, christophe longuet10, subbalaxmi vs malladi9, deepali mukerji5, Deepthi Nair11, alamelu raja12, balambal raman12, camilla rodrigues13, pratibha sharma11, amit singh3, sarman singh8, archana sodha13, basirudeen syed ahamed kabeer12 guy vernet10, delia goletti6**. Accepted for publication in Plos one July 2012.
  • Smear positivity- to be or not tb? Aditya Singh, Deepthi Nair, Deepti Rawat. Monograph. Lambert. (48pages) April 2012.
  • Clinical utility of combined cerebrospinal fluid culture and real time pcr in pediatric tubercular meningitis – (neuped-01-2012-0785-sc) Mukul Agarwal,Deepthi Nair, KC Agarwal. Accepted Neuropediatrics 2012
  • J. Grenier, l. Pinto, D. Nair, K. Steingart, D. Dowdy, A. Ramsay, and M. Pai. Widespread use of serological tests for tuberculosis: data from 22 high-burden countries. Eur respir J 2012 39:502-505
  • Shariff M,Chowdhary J,Zahroor S, Deb M.Characterization Of Streptococcus pneumoniae isolates from India with special reference to their sequence types. . J. Infect Dev. Countries 2013;7(2)101-109.
  • Community acquisition of β-lactamase producing Enterobateriacease in neonatal gut Authors : Kothari Charu, Gaind Rajni, Singh Chandreshwor Laishram, Sinha Anju, Kumari Vidya, Arya Sugandha, Chellani Harish, Saxena Sunita, Deb Monorama, DOI: 10.1186/1471-2180-13-136

Publications in National Journals

  • Bala M, Toor A, Malhotra M, Kakran M, Muralidhar S, Ramesh V. Evaluation of the usefulness of Treponema pallidum haemagglutination test in the diagnosis of syphilis in weak reactive VDRL sera. Indian J Sex Transm Dis. 2012:33(2):102-6.
  • Sood S, Mukherjee A, Bala M, Satpathy G, Mahajan N, Sharma A, et al. A pilot study for diagnosis of genital Chlamydia trachomatis infections by polymerase chain reaction among symptomatic Indian women. Indian J Dermatol Venereol Leprol. 2012;78(4): 443-7.
  • Jain R, Muralidhar S, Talwar R. Sexual resilience within intimate relations among unmarried adolescent girls in Delhi, India. Accepted by Indian Journal of Sexually Transmitted Diseases & AIDS.Dec 2012.
  • Mohapatra S, Panda P, Parida B. Genital tract infection of women in Southern Orissa with special reference to pelvic inflammatory disease. Indian Journal of sexually Transmitted Disease and AIDS ,2013; 34;64-66
  • Sarika Jain, Sudha Chandelia. A CASE OF MULTIPLE BRAIN ABSCESSES DUE TO STAPHYLOCOCCUS AUREUS SECONDARY TO CONGENITAL DACRYOCYSTITIS. Accepted for publication in Ind J Med Sc 2013. Manuscript ID No: IJMS-371-12.
  • Jain S, Singh S, Shankar MM, Saha R, Rao R, Chugh TD. Catheter related bacteremia due to M. chelonae in an immunocompromised patient- an emerging nosocomial pathogen. Indian Journal of Medical Microbiology 2012;30(4):489-91
  • Jain S, Arpra S, Saha R, Kaur IR. Serratia plymuthica: a community acquired uropathogen. Indian J Med SC 2012 (accepted for publication).
  • Jain S, Bohra I, Mahajan R, Jain S, Chugh TD. Pantoea agglomerans infection behaving as tumour after plant thorn injury. Indian J Pathol Microbiol 2012; 55(3): 386-8.
  • 'Use of amplified mycobacterium tuberculosis direct test { genprobe(inc , san diego , ca)} in diagnosis of early tubercular arthritis of knee joint'. V Agarwal, D. Nair, G Khanna, S Batra, VK Sharma. Indian J orthoped 2012 ; 46( 5): 83-87.
  • Rapid culture diagnosis of tuberculous lymphadenitis from a tertiary care centre in an endemic nation: potential and pitfalls. JVerma, D Kasana, D Nair. IJMM July-Sept 2012.
  • Shariff M, Zahoor S, Deb M. Serotype 19A Streptococcus pneumonia among Indian Isolates is a cause for concern. Indian J of Med Microbiol.2012;(1):112
  • Chugh S, Kaur J, Deb M. Resistance spectrum of antimicrobial among community acquired uropathogens in a tertiary care hospital in Delhi. J Communicable Dis .2012;43(3):2011-17
  • Neonatal septicaemia caused by diverse clones of Klebsiella pneumonia & Escherichia coli harbouring blaCTX-M-15. Subhasree Roy, Rajni Gaind, Harish Chellani, Srujana Mohanty, Saswati Datta, Arun K. Singh & Sulagna Basu. Indian J Med Res 137,April 2013 , pp 791-799
  • Balvinder Singh Arora, Sonia Chugh, B. Gupta and K.C Agrawal. Dengue and Chikungunya virus fever outbreaks in Delhi, IgM Serology status – A recent experience. National Journal of Basic Medical Sciences 2011; II (4);336-40.

Contribution in Books (Jan 2010 to Dec 2012)

  • Dr. Sumathi Muralidhar contributed chapter on Hospital Acquired Infections for book on Patient Safety compiled by IGNOU for Ministry of Health & Family Welfare, Nirman Bhavan, New Delhi. 2010
  • Book Chapter: Dr Rajni Gaind Chapter ‘ Prevention of Infections in Nursery in the book titled “Essential Neonatology” pg 47-63 Editors . NB Mathur Edition 2012, Noble Vision New Delhi.
  • Book/ Chapter in a Book (publication): Chapter on Fungal Surgical Site Infections: Malini R Capoor, in Book by Jaypee..2012.
  • Text Book of microbiology , Globalmedilink, a Health Science Publisher 2100, First Edition Pg 622 ISHN 978-81-904011-8-0

  • MEDISCON 2012 at VMMC, New Delhi , 9-11 August 2012. All faculty
  • Safe Hands Symposiums, at Bangkok, 20-22 March 2012, Dr. Dimple Kasana presented a paper (NSIP in health care workers in ICU of a Tertiary Care Hospital)
  • Emerging and Remerging infections conference (CDC-NDMA FICCI) at IHC, Delhi, May 2013 attended Dr. Malini R. Capoor, Dr. Deepthi Nair, Dr. Dimple Kasana.
  • MICRO-D-CON 2012 4th Annual conference on “Bioinformatics in Microbiology” organized by Indian Association of Medical Microbiologist Delhi Chapter attended by Dr. Monorama Deb, Dr. Jagdev Kaur.
  • MICROCON – 2012, Conference and CME (IAMM) organized by Department of Microbiology, LHMC, New Delhi attended by Dr. Monorama Deb, Dr. Jagdev Kaur, Dr. Deepthi Nair, Dr. Dimple Kasana, Dr. Malini R. Capoor, Dr. Sudhir K. Bagdi
  • Delhi Chapter IAMM Annual Conference AIIMS 26th Mar 2012.
  • RNTCP North Zone- Annual National Conference Feb 2012, MAMC 
     
    • Adjudged the best participating Medical College
    • Best Paper Award First Prize- Molecular tests in Pleural Tuberculosis
  • 13th IUSTI World Congress at Australia from 15th to 17th October 2012.
  • 36th National Conference of Indian Association for the study of STDs & AIDS held from 2nd to 4th November 2012 Kochi Dr Manju Bala.
  • 12th National Conference on Prevention & Control of Healthcare Associated Infections held on 8th & 9th February , at Tata Memorial Hospital,Parel, Mumbai. Dr. Rajni Gaind.
  • 11. 10-12 Feb 2012, National Conference SIHAM, Silliguri. Dr. Malini Capoor

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  • MBBS
  • Post Graduate (Microbiology)

  • B.Sc. (Nursing)

    • College of Nursing
    • IGNOU
    • DMLT
  • Certificate courses for Medical Record Officers, Technicians & Pre-Hospital Trauma Technicians.

Awards:

  • RNTCP North Zone- Annual National Conference Feb 2012, MAMC

    • Adjudged the best participating Medical College
    • Best Paper Award First Prize- Molecular tests in Pleural Tuberculosis Ms Pratibha Sharma
  • Panfungal RT-PCR in diagnosis of Invasive fungal infections. 10-12 Feb 2012, National Conference SIHAM, Silliguri (Best Poster Prize) &ECMM T Award. Dr Malini capoor.
  • Delhi Chapter IAMM Annual Conference AIIMS 26th Mar 2012. Evaluations of alterations in gidB region in streptomycin resistant Mycobacterium tuberculosis isolates. First Prize in Oral Young scientist award. J Verma
  • Received Dr PS Ranganathan Memorial Award -3rd prize for paper presented on “Investigating seasonal variations in gonorrhoea using graphical and ratio- to-moving average methods” at 36th National Conference of Indian Association for the study of STDs & AIDS held from 2nd to 4th November 2012 at Kochi. Dr. Manju Bala
  • Received 2nd prize for the paper outbreak of Proteus mirabilis produanp VEB-extended spectrum β lactamase gene is neonatal ICU. Presented at 12th National Conference on Prevention & Control of Healthcare Associated Infections held on 8th & 9th February, at Tata Memorial Hospital,Parel, Mumbai. Dr. Rajni Gaind.

At present there is no content available for this section, once content will be available would be updated.